PERMEABILITY OF HYDROPHILIC MODEL DRUGS MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS

MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS SUMMARY Investigations of the integrity and transport characteristics of 2/4/A1 cells have been done in this report. The cell line was isolated from rat fetal intestinal epithelial cells and transfected with thermolabile SV40 large T antigen. These cells proliferated at 33 °C, but eliminated the antigen and ceased proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells started to differentiate but simultaneously the cells also underwent massive …showed first 75 words of 3421 total…

…showed last 75 words of 3421 total…Conditionally immortalized intestinal epithelial cells: novel approach for study of differentiated enterocytes. Am J Physiol 1993; 265: C266-78 Walter E, Kissel T. Heterogenity in the human intestinal cell line Caco-2 leads to differences in epithelial transport. Eur J Pharm. Sci 1995; 3: 215-30 Whitehead RH, VanEeden PE, Noble MD, Ataliotis MD, Jat PS. Establishment of conditionally immortalized epithelial cell lines from both colon and small intestine of adult H-2Kb-tsA58 transgenic mice. Proc Natl Acad Sci USA 1993; 90:587-591