Knock out mutants

Basic summary on knock-outs In vitro, a DNA fragment is altered by insertion of a fragment containing a selectable marker. The interrupted gene is introduced into the cells. In some cells, homologous recombination results in the replacement of the resident “gene” with the interrupted gene. Homozygous transgenic cells are often referred to as “knock-outs”. These knock-outs can then be examined to determine what, if any, phenotype is associated with mutations in the targeted gene. If …showed first 75 words of 672 total…

…showed last 75 words of 672 total…single restriction site of the rpoS cloned in the low copy number plasmid (B), which has ends compatible with EcoRI. The resulting plasmid (X) can then be confirmed to contain the rpoS::selectable marker construct by DNA sequencing. Sources used: Elias, F. Abdallah, James L. Bono,James A. Carroll, Philip Stewart, Kit Tilly, and Patricia Rosa. 2000. Altered Stationary-Phase Response in a Borrelia burgdorferi rpoS Mutant. Journal of Bacteriology, May 2000, p. 2909-2918, Vol. 182, No. 10 ------------------------------------------------------------------------ **Bibliography**